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Anti-Smarcd1 Antibody
Catalog #: 144-09950
Smarcd1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 83797 |
| Gene Symbols | SMARCD1 |
| Protein Name / Synonyms | SMARCC1, BAF155, CRACC1, Rsc8, SRG3, SWI3, SWI/SNF complex subunit SMARCC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
58kDa
Observed MW
58kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of mouse BAF60a (NP_114030.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Smarcd1 antibody (144-09950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (144-09950) (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (144-09950) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (144-09950) (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (144-09950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (144-09950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (144-09950) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.