Anti-SMARCE1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q969G3
Gene Id	6605
Gene Symbols	SMARCE1
Protein Name / Synonyms	SMARCE1, BAF57, CSS5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50

Format

Calculated MW

34kDa/37kDa/39kDa/41kDa/42kDa/46kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (144-602973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)

Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (144-602973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)

Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (144-602973).
Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)

Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (144-602973). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (144-602973) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)

Expiration:

12 months from the date of shipment when stored properly.

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