3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SMCHD1 Antibody
Catalog #: 144-07214
SMCHD1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | A6NHR9 |
| Gene Id | 23347 |
| Gene Symbols | SMCHD1 |
| Protein Name / Synonyms | SMCHD1, BAMS, FSHD2, structural maintenance of chromosomes flexible hinge domain containing 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
85kDa/215kDa/226kDa
Observed MW
226kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1756-2005 of human SMCHD1 (NP_056110.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMCHD1 antibody (144-07214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using SMCHD1 antibody (144-07214). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.