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Anti-Smooth Muscle Actin Antibody
Catalog #: 188-10002
Mouse anti-Human/Baboon/Monkey/Cow/Pig/Sheep/Goat/Cat/Dog/Rabbit/Mouse/Rat/Guinea pig/Chicken. Smooth Muscle Actin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Mouse |
| Accession Number | P62736 |
| Gene Id | 59 |
| Gene Symbols | ACTA2,ACTSA,ACTVS,GIG46 |
| Protein Name / Synonyms | Actin, aortic smooth muscle (Alpha-actin-2) (Cell growth-inhibiting gene 46 protein) |
| Immunogen | An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody. |
| Isotype | Kappa, IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Western blot: 0.5-1ug/ml FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml,IHC(FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
Storage
Store the Smooth Muscle Actin antibody at 2-8°C (aliquot/freeze the azide-free format).
Expiration:
12 months from the date of shipment when stored properly.