Anti-SMPD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P17405
Gene Id	6609
Gene Symbols	SMPD1
Protein Name / Synonyms	SMPD1, ASM, ASMASE, NPD, sphingomyelin phosphodiesterase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

63kDa/64kDa/69kDa

Observed MW

60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 (NP_000534.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SMPD1 antibody (144-06743) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - SMPD1 antibody (144-06743)

Immunohistochemistry of paraffin-embedded rat ovary using SMPD1 antibody (144-06743) at dilution of 1:100 (40x lens).
Immunohistochemistry - SMPD1 antibody (144-06743)

Immunofluorescence analysis of HeLa cells using SMPD1 antibody (144-06743). Blue: DAPI for nuclear staining.
Immunofluorescence - SMPD1 antibody (144-06743)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products