SMYD1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8NB12 |
| Gene Id | 150572 |
| Gene Symbols | SMYD1 |
| Protein Name / Synonyms | SMYD1, BOP, KMT3D, ZMYND18, ZMYND22, SET and MYND domain containing 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:200 - 1:2000
IHC 1:20 - 1:200
IF 1:20 - 1:100
IHC 1:20 - 1:200
IF 1:20 - 1:100
Format
Calculated MW
56kDa
Observed MW
57kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SMYD1 (NP_938015.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMYD1 Antibody (144-02340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using SMYD1 antibody (144-02340) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using SMYD1 antibody (144-02340) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SMYD1 antibody (144-02340) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SMYD1 antibody (144-02340). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
