RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-SMYD2 Antibody

Catalog #: 144-06474

SMYD2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9NRG4
Gene Id
56950
Gene Symbols
SMYD2
Protein Name / Synonyms
SMYD2, HSKM-B, KMT3C, ZMYND14, N-lysine methyltransferase SMYD2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW
30kDa/49kDa
Observed MW
50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SMYD2 antibody (144-06474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - SMYD2 Polyclonal Antibody (144-06474)

Immunofluorescence analysis of U2OS cells using SMYD2 antibody (144-06474). Blue: DAPI for nuclear staining.
Immunofluorescence - SMYD2 Polyclonal Antibody (144-06474)

Expiration:
12 months from the date of shipment when stored properly.