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Anti-SMYD4 Antibody
Catalog #: 144-07310
SMYD4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8IYR2 |
| Gene Id | 114826 |
| Gene Symbols | SMYD4 |
| Protein Name / Synonyms | SMYD4, ZMYND21, SET and MYND domain containing 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
89kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMYD4 antibody (144-07310) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human gastric cancer using SMYD4 antibody (144-07310) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse jejunum using SMYD4 antibody (144-07310) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using SMYD4 antibody (144-07310). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.