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Anti-SNAP29 Antibody
Catalog #: 144-04290
SNAP29 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95721 |
| Gene Id | 9342 |
| Gene Symbols | SNAP29 |
| Protein Name / Synonyms | SNAP29, CEDNIK, SNAP-29, synaptosome associated protein 29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.
Dilutions
WB 1:1000 - 1:4000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
28kDa
Observed MW
35kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNAP29 (NP_004773.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SNAP29 antibody (144-04290) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver injury using SNAP29 antibody (144-04290) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SNAP29 antibody (144-04290) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SNAP29 antibody (144-04290) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SNAP29 antibody (144-04290) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.