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Anti-SOX5 Antibody
Catalog #: 144-06985
SOX5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35711 |
| Gene Id | 6660 |
| Gene Symbols | SOX5 |
| Protein Name / Synonyms | SOX5, L-SOX5, L-SOX5B, L-SOX5F, LAMSHF, SRY-box 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
41kDa/71kDa/82kDa/84kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SOX5 (NP_694534.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SOX5 antibody (144-06985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (144-06985) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (144-06985) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (144-06985) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (144-06985) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (144-06985) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SOX5 antibody (144-06985).
Expiration:
12 months from the date of shipment when stored properly.