Anti-SOX9 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P48436
Gene Id	6662
Gene Symbols	SOX9
Protein Name / Synonyms	SOX9, CMD1, CMPD1, SRA1, SRXX2, SRXY10, SRY-box 9
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

56kDa

Observed MW

60kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SOX9 (NP_000337.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SOX9 antibody (144-02479) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.
Western blot - SOX9 Polyclonal Antibody (144-02479)

Immunofluorescence analysis of HeLa cells using SOX9 antibody (144-02479) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - SOX9 Polyclonal Antibody (144-02479)

Expiration:

12 months from the date of shipment when stored properly.

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