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Anti-SPHK1 Antibody
Catalog #: 144-00139
SPHK1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9NYA1 |
| Gene Id | 8877 |
| Gene Symbols | SPHK1 |
| Protein Name / Synonyms | SPHK1, SPHK, sphingosine kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
42kDa/43kDa/51kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SPHK1 antibody (144-00139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric cancer using SPHK1 Antibody (144-00139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibody (144-00139) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibody (144-00139) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SPHK1 Antibody (144-00139) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.