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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P16150 |
| Gene Id | 6693 |
| Gene Symbols | SPN |
| Protein Name / Synonyms | SPN, CD43, GALGP, GPL115, LSN, leukosialin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
40kDa
Observed MW
130kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 276-400 of human SPN (NP_003114.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of THP-1 cells, using SPN antibody (144-06412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SPN antibody (144-06412) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.