Anti-SRPK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q96SB4
Gene Id	6732
Gene Symbols	SRPK1
Protein Name / Synonyms	SRPK1, SFRSK1, SRSF protein kinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

72kDa/74kDa/92kDa

Observed MW

70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 290-480 of human SRPK1 (NP_003128.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SRPK1 antibody (144-05854) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - SRPK1 antibody (144-05854)

Immunohistochemistry of paraffin-embedded human kidney cancer using SRPK1 antibody (144-05854) at dilution of 1:200 (40x lens).
Immunohistochemistry - SRPK1 antibody (144-05854)

Immunofluorescence analysis of HeLa cells using SRPK1 antibody (144-05854). Blue: DAPI for nuclear staining.
Immunofluorescence - SRPK1 antibody (144-05854)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products