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Anti-SSB Antibody
Catalog #: 144-00630
SSB Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P05455 |
| Gene Id | 6741 |
| Gene Symbols | SSB |
| Protein Name / Synonyms | SSB, LARP3, La, La/SSB, lupus La protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
46kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SSB antibody (144-00630) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver cancer using SSB antibody (144-00630) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using SSB antibody (144-00630) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using SSB antibody (144-00630) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SSB antibody (144-00630) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SSB antibody (144-00630) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.