3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SSRP1 Antibody
Catalog #: 144-60423
SSRP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q08945 |
| Gene Id | 6749 |
| Gene Symbols | SSRP1 |
| Protein Name / Synonyms | SSRP1, FACT, FACT80, T160, FACT complex subunit SSRP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
81kDa
Observed MW
85kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SSRP1 (NP_003137.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SSRP1 antibody (144-013636) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using SSRP1 antibody (144-013636) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SSRP1 antibody (144-013636).
Expiration:
12 months from the date of shipment when stored properly.