RayBiotech

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Anti-ST3GAL5 Antibody

Catalog #: 144-07947

ST3GAL5 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9UNP4
Gene Id
8869
Gene Symbols
ST3GAL5
Protein Name / Synonyms
ST3GAL5, SATI, SIAT9, SIATGM3S, SPDRS, ST3GalV, lactosylceramide alpha-2,3-sialyltransferase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
45kDa/47kDa
Observed MW
48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human ST3GAL5 (NP_003887.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ST3GAL5 antibody (144-07947) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - ST3GAL5 antibody (144-07947)

Expiration:
12 months from the date of shipment when stored properly.