ST8SIA2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q92186 |
| Gene Id | 8128 |
| Gene Symbols | ST8SIA2 |
| Protein Name / Synonyms | ST8SIA2, HsT19690, SIAT8-B, SIAT8B, ST8SIA-II, ST8SiaII, STX, alpha-2,8-sialyltransferase 8B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
42kDa
Observed MW
42kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human ST8SIA2 (NP_006002.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ST8SIA2 antibody (144-07748) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using ST8SIA2 antibody (144-07748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ST8SIA2 antibody (144-07748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ST8SIA2 antibody (144-07748) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.