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Anti-STAT1 Antibody
Catalog #: 144-00027
STAT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42224 |
| Gene Id | 6772 |
| Gene Symbols | STAT1 |
| Protein Name / Synonyms | STAT1, CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91, signal transducer and activator of transcription 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
83kDa/87kDa
Observed MW
90kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 513-712 of human STAT1 (NP_644671.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using STAT1 antibody (144-00027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using STAT1 antibody (144-00027) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using STAT1 antibody (144-00027) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.