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Anti-STK11 Antibody
Catalog #: 144-02122
STK11 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15831 |
| Gene Id | 6794 |
| Gene Symbols | STK11 |
| Protein Name / Synonyms | STK11, LKB1, PJS, hLKB1, serine/threonine kinase 11 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
45kDa/48kDa
Observed MW
54kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human STK11 (NP_000446.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using STK11 antibody (144-02122) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using STK11 antibody (144-02122) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using STK11 antibody (144-02122) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using STK11 antibody (144-02122) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using STK11 antibody (144-02122) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using STK11 antibody (144-02122) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using STK11 antibody (144-02122). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.