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Anti-STK3 Antibody
Catalog #: 144-06992
STK3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13188 |
| Gene Id | 6788 |
| Gene Symbols | STK3 |
| Protein Name / Synonyms | STK3, KRS1, MST2, serine/threonine kinase 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
56kDa/59kDa
Observed MW
65kDa, 37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 323-450 of human STK3 (NP_006272.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using STK3 antibody (144-06992) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using STK3 antibody (144-06992) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using STK3 antibody (144-06992) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using STK3 antibody (144-06992) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using STK3 antibody (144-06992) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.