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Anti-STK4 Antibody
Catalog #: 144-08043
STK4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13043 |
| Gene Id | 6789 |
| Gene Symbols | STK4 |
| Protein Name / Synonyms | STK4, KRS2, MST1, YSK3, serine/threonine kinase 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
52kDa/55kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human STK4 (NP_006273.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using STK4 antibody (144-08043) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot analysis of extracts of various cells, using STK4 antibody (144-08043) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody (144-08043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody (144-08043) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.