RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-STX12 Antibody

Catalog #: 144-08200

STX12 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q86Y82
Gene Id
23673
Gene Symbols
STX12
Protein Name / Synonyms
STX12, STX13, STX14, syntaxin-12
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaAutophagy
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
31kDa
Observed MW
31kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human STX12 (NP_803173.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using STX12 antibody (144-08200) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - STX12 antibody (144-08200)

Immunofluorescence analysis of U2OS cells using STX12 antibody (144-08200) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - STX12 antibody (144-08200)

Expiration:
12 months from the date of shipment when stored properly.