STXBP1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P61764 |
| Gene Id | 6812 |
| Gene Symbols | STXBP1 |
| Protein Name / Synonyms | STXBP1, MUNC18-1, NSEC1, P67, RBSEC1, UNC18, syntaxin-binding protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
67kDa/68kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using STXBP1 antibody (144-05420) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (144-05420) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (144-05420) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using STXBP1 antibody (144-05420) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using STXBP1 antibody (144-05420) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using STXBP1 antibody (144-05420) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using STXBP1 antibody (144-05420) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.