Anti-SUFU Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UMX1
Gene Id	51684
Gene Symbols	SUFU
Protein Name / Synonyms	SUFU, PRO1280, SUFUH, SUFUXL, suppressor of fused homolog
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

47kDa/52kDa/53kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SUFU antibody (144-06757) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - SUFU Polyclonal Antibody (144-06757)

Immunohistochemistry of paraffin-embedded human stomach using SUFU antibody (144-06757) at dilution of 1:100 (40x lens).
Immunohistochemistry - SUFU Polyclonal Antibody (144-06757)

Immunofluorescence analysis of MCF7 cells using SUFU antibody (144-06757). Blue: DAPI for nuclear staining.
Immunofluorescence - SUFU Polyclonal Antibody (144-06757)

Expiration:

12 months from the date of shipment when stored properly.

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