RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-SULT1E1 Antibody

Catalog #: 144-07452

SULT1E1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P49888
Gene Id
6783
Gene Symbols
SULT1E1
Protein Name / Synonyms
SULT1E1, EST, EST-1, ST1E1, STE, estrogen sulfotransferase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
35kDa
Observed MW
35kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 140-294 of human SULT1E1 (NP_005411.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SULT1E1 antibody (144-07452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - SULT1E1 Polyclonal Antibody (144-07452)

Immunofluorescence analysis of A549 cells using SULT1E1 antibody (144-07452). Blue: DAPI for nuclear staining.
Immunofluorescence - SULT1E1 Polyclonal Antibody (144-07452)

Expiration:
12 months from the date of shipment when stored properly.