SULT2B1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00204 |
| Gene Id | 6820 |
| Gene Symbols | SULT2B1 |
| Protein Name / Synonyms | SULT2B1, HSST2, sulfotransferase family 2B member 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
39kDa/41kDa
Observed MW
41kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-365 of human SULT2B1 (NP_814444.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SULT2B1 antibody (144-07736) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using SULT2B1 antibody (144-07736) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SULT2B1 antibody (144-07736) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SULT2B1 antibody (144-07736) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SULT2B1 antibody (144-07736) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SULT2B1 antibody (144-07736).
Expiration:
12 months from the date of shipment when stored properly.