RayBiotech

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ISO 13485:2016 cGMP

Anti-SUPT20H Antibody

Catalog #: 144-07374

SUPT20H Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q8NEM7
Gene Id
55578
Gene Symbols
SUPT20H
Protein Name / Synonyms
SUPT20H, C13, C13orf19, FAM48A, FP757, P38IP, SPT20, transcription factor SPT20 homolog
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Research AreaAutophagy
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
80kDa/85kDa/88kDa
Observed MW
86kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 580-779 of human SUPT20H (NP_001014308.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SUPT20H antibody (144-07374) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - SUPT20H Polyclonal Antibody (144-07374)

Immunohistochemistry of paraffin-embedded human colon using SUPT20H antibody (144-07374) at dilution of 1:100 (40x lens).
Immunohistochemistry - SUPT20H Polyclonal Antibody (144-07374)

Immunohistochemistry of paraffin-embedded human kidney using SUPT20H antibody (144-07374) at dilution of 1:100 (40x lens).
Immunohistochemistry - SUPT20H Polyclonal Antibody (144-07374)

Immunohistochemistry of paraffin-embedded mouse liver using SUPT20H antibody (144-07374) at dilution of 1:100 (40x lens).
Immunohistochemistry - SUPT20H Polyclonal Antibody (144-07374)

Immunofluorescence analysis of A549 cells using SUPT20H antibody (144-07374). Blue: DAPI for nuclear staining.
Immunofluorescence - SUPT20H Polyclonal Antibody (144-07374)

Expiration:
12 months from the date of shipment when stored properly.