Anti-SUZ12 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15022
Gene Id	23512
Gene Symbols	SUZ12
Protein Name / Synonyms	SUZ12, CHET9, JJAZ1, polycomb protein SUZ12
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

83kDa

Observed MW

83kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (144-07786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (144-07786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody (144-07786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (144-07786) at a dilition of 1:500.
Immunoprecipitation - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)

Expiration:

12 months from the date of shipment when stored properly.

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