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Anti-SYNCRIP Antibody
Catalog #: 144-07219
SYNCRIP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60506 |
| Gene Id | 10492 |
| Gene Symbols | SYNCRIP |
| Protein Name / Synonyms | SYNCRIP, GRY-RBP, GRYRBP, HNRNPQ, HNRPQ1, NSAP1, PP68, hnRNP-Q, heterogeneous nuclear ribonucleoprotein Q |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 20.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
46kDa/58kDa/62kDa/65kDa/69kDa
Observed MW
50-100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SYNCRIP (NP_001153146.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SYNCRIP antibody (144-07219) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver cancer using SYNCRIP antibody (144-07219) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using SYNCRIP antibody (144-07219) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SYNCRIP antibody (144-07219) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using SYNCRIP antibody (144-07219). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.