Anti-TADA2A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O75478
Gene Id	6871
Gene Symbols	TADA2A
Protein Name / Synonyms	TADA2A, ADA2, ADA2A, KL04P, TADA2L, hADA2, transcriptional adaptor 2A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. Several alternatively spliced transcript variants encoding different isoforms of this gene have been described, but the full-length nature of some of these variants has not been determined.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

36kDa/51kDa

Observed MW

52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-443 of human TADA2A (NP_001159577.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TADA2A antibody (144-08457) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Western blot - TADA2A Polyclonal Antibody (144-08457)

Expiration:

12 months from the date of shipment when stored properly.

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