Anti-TADA3L (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75528
Gene Id	10474
Gene Symbols	TADA3
Protein Name / Synonyms	TADA3L (C-term)
Clonality	Polyclonal
Preparation	This TADA3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human TADA3L.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq].

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus

Other Names

TADA3; ADA3; TADA3L; Transcriptional adapter 3; ADA3 homolog; STAF54; Transcriptional adapter 3-like

NCBI Accession #

NP_001265199.1;NP_006345.1;NP_597814.1

Other Accession #

Q4V8F5; Q8R0L9; Q7SY21; Q5EAE2; Q66IZ5; Q6PGT0; NP_006345.1; NP_597814.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48902

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

TADA3L Antibody (C-term) (Cat# 102-12642) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).

Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term)(Cat# 102-12642).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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