RayBiotech

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ISO 13485:2016 cGMP

Anti-TAF15 Antibody

Catalog #: 144-08465

TAF15 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q92804
Gene Id
8148
Gene Symbols
TAF15
Protein Name / Synonyms
TAF15, Npl3, RBP56, TAF2N, TAFII68, TATA-binding protein-associated factor 2N
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
61kDa
Observed MW
80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TAF15 (NP_631961.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TAF15 antibody (144-08465) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 5s.
Western blot - TAF15 antibody (144-08465)

Immunohistochemistry of paraffin-embedded human stomach using TAF15 antibody (144-08465) at dilution of 1:100 (40x lens).
Immunohistochemistry - TAF15 antibody (144-08465)

Immunohistochemistry of paraffin-embedded human esophagus using TAF15 antibody (144-08465) at dilution of 1:100 (40x lens).
Immunohistochemistry - TAF15 antibody (144-08465)

Expiration:
12 months from the date of shipment when stored properly.