Anti-TAF9 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q16594
Gene Id	6880
Gene Symbols	TAF9
Protein Name / Synonyms	TAF9 (N-term)
Clonality	Polyclonal
Preparation	This TAF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TAF9.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants encoding different isoforms.

Specificity

Other Names

TAF9; TAF2G; TAFII31; Transcription initiation factor TFIID subunit 9; RNA polymerase II TBP-associated factor subunit G; STAF31/32; Transcription initiation factor TFIID 31 kDa subunit; Transcription initiation factor TFIID 32 kDa subunit

NCBI Accession #

NP_001015892.1;NP_003178.1

Other Accession #

NP_001015892.1; NP_003178.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28974

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of U251 cell stained with TAF9 Antibody (N-term)(Cat# 102-12563).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TAF9 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).TAF9 immunoreactivity is localized to Nucleus significantly.

TAF9 Antibody (N-term) (Cat# 102-12563) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TAF9 antibody detected the TAF9 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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