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Anti-TARS Antibody
Catalog #: 144-06993
TARS Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P26639 |
| Gene Id | 6897 |
| Gene Symbols | TARS |
| Protein Name / Synonyms | TARS, ThrRS, threonyl-tRNA synthetase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
83kDa/86kDa
Observed MW
83kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TARS (NP_689508.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TARS Antibody (144-06993) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse kidney using TARS antibody (144-06993) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TARS antibody (144-06993) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TARS antibody (144-06993) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TARS antibody (144-06993). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.