Anti-TAT Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P17735
Gene Id	6898
Gene Symbols	TAT
Protein Name / Synonyms	TAT, tyrosine aminotransferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible mental retardation. A regulator gene for tyrosine aminotransferase is X-linked.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

50kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TAT (NP_000344.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TAT antibody (144-06764) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.
Western blot - TAT antibody (144-06764)

Immunofluorescence analysis of MCF-7 cells using TAT antibody (144-06764). Blue: DAPI for nuclear staining.
Immunofluorescence - TAT antibody (144-06764)

Expiration:

12 months from the date of shipment when stored properly.

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