Anti-TBX6 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O95947
Gene Id	6911
Gene Symbols	TBX6
Protein Name / Synonyms	TBX6 (Center)
Clonality	Polyclonal
Preparation	This TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the Central region of human TBX6.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

TBX6; T-box transcription factor TBX6

NCBI Accession #

NP_004599.2

Other Accession #

D3ZJK7; P70327; E1BEA8; NP_004599.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

47045

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

TBX6 Antibody (Center) (Cat# 102-11379) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).

Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center)(Cat# 102-11379). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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