RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-TBXAS1 Antibody

Catalog #: 144-60304

TBXAS1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P24557
Gene Id
6916
Gene Symbols
TBXAS1
Protein Name / Synonyms
TBXAS1, BDPLT14, CYP5, CYP5A1, GHOSAL, THAS, TS, TXAS, TXS, thromboxane-A synthase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
52kDa/60kDa/65kDa
Observed MW
50kDa, 60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 355-534 of human TBXAS1 (NP_001052.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TBXAS1 antibody (144-60304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - TBXAS1 antibody (144-60304)

Immunohistochemistry of paraffin-embedded mouse heart using TBXAS1 antibody (144-60304) at dilution of 1:100 (40x lens).
Immunohistochemistry - TBXAS1 antibody (144-60304)

Immunofluorescence analysis of HeLa cells using TBXAS1 antibody (144-60304). Blue: DAPI for nuclear staining.
Immunofluorescence - TBXAS1 antibody (144-60304)

Expiration:
12 months from the date of shipment when stored properly.