Anti-TCN2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P20062
Gene Id	6948
Gene Symbols	TCN2
Protein Name / Synonyms	TCN2, D22S676, D22S750, II, TC, TC II, TC-2, TC2, TCII, transcobalamin-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

44kDa/47kDa

Observed MW

54kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-250 of human TCN2 (NP_001171655.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TCN2 antibody (144-05755) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - TCN2 Polyclonal Antibody (144-05755)

Immunofluorescence analysis of U2OS cells using TCN2 antibody (144-05755). Blue: DAPI for nuclear staining.
Immunofluorescence - TCN2 Polyclonal Antibody (144-05755)

Expiration:

12 months from the date of shipment when stored properly.

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