Anti-TCP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P17987
Gene Id	6950
Gene Symbols	TCP1
Protein Name / Synonyms	TCP1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, t-complex 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

60kDa

Observed MW

60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TCP1 antibody (144-60306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - TCP1 antibody (144-60306)

Immunofluorescence analysis of U2OS cells using TCP1 antibody (144-60306). Blue: DAPI for nuclear staining.
Immunofluorescence - TCP1 antibody (144-60306)

Expiration:

12 months from the date of shipment when stored properly.

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