RayBiotech

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Anti-TEAD3 Antibody

Catalog #: 144-07454

TEAD3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q99594
Gene Id
7005
Gene Symbols
TEAD3
Protein Name / Synonyms
TEAD3, DTEF-1, ETFR-1, TEAD-3, TEAD5, TEF-5, TEF5, TEA domain transcription factor 3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and is involved in the transactivation of the chorionic somatomammotropin-B gene enhancer. Translation of this protein is initiated at a non-AUG (AUA) start codon.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
48kDa
Observed MW
49kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TEAD3 antibody (144-07454) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TEAD3 Polyclonal Antibody (144-07454)

Immunohistochemistry of paraffin-embedded human colon using TEAD3 antibody (144-07454) at dilution of 1:100 (40x lens).
Immunohistochemistry - TEAD3 Polyclonal Antibody (144-07454)

Immunofluorescence analysis of A549 cells using TEAD3 antibody (144-07454).
Immunofluorescence - TEAD3 Polyclonal Antibody (144-07454)

Expiration:
12 months from the date of shipment when stored properly.