Anti-TFRC Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P02786
Gene Id	7037
Gene Symbols	TFRC
Protein Name / Synonyms	TFRC, CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90, transferrin receptor
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.05% proclin300, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

84kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TFRC (NP_001121620.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TFRC antibody (144-05865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - TFRC antibody (144-05865)

Immunohistochemistry of paraffin-embedded human placenta using TFRC antibody (144-05865) at dilution of 1:100 (40x lens).
Immunohistochemistry - TFRC antibody (144-05865)

Expiration:

12 months from the date of shipment when stored properly.

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