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Anti-TGFB2 Antibody
Catalog #: 144-03640
TGFB2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P61812 |
| Gene Id | 7042 |
| Gene Symbols | TGFB2 |
| Protein Name / Synonyms | TGFB2, G-TSF, LDS4, TGF-beta2, transforming growth factor beta-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.01% thimerosal, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis, Cardiovascular Disease, TGF-beta Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:200 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
47kDa/50kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human TGFB2 (NP_001129071.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TGFB2 antibody (144-03640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.