TGM5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43548 |
| Gene Id | 9333 |
| Gene Symbols | TGM5 |
| Protein Name / Synonyms | TGM5, PSS2, TGASE5, TGASEX, TGM6, TGMX, TGX, transglutaminase 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the transglutaminase family. The encoded protein catalyzes formation of protein cross-links between glutamine and lysine residues, often resulting in stabilization of protein assemblies. This reaction is calcium dependent. Mutations in this gene have been associated with acral peeling skin syndrome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
71kDa/80kDa
Observed MW
81kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TGM5 antibody (144-07039) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat kidney using TGM5 antibody (144-07039) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using TGM5 antibody (144-07039) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TGM5 antibody (144-07039) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.