Anti-TH Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P07101
Gene Id	7054
Gene Symbols	TH
Protein Name / Synonyms	TH, DYT14, DYT5b, TYH, tyrosine hydroxylase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Dilutions

WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Format

Calculated MW

44kDa/45kDa/55kDa/56kDa/58kDa

Observed MW

60kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TH (NP_954986.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TH antibody (144-00028) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - TH Polyclonal Antibody (144-00028)

Immunohistochemistry of paraffin-embedded rat brain using TH Antibody (144-00028) at dilution of 1:100 (40x lens).
Immunohistochemistry - TH Polyclonal Antibody (144-00028)

Immunohistochemistry of paraffin-embedded mouse spinal cord using TH Antibody (144-00028) at dilution of 1:100 (40x lens).
Immunohistochemistry - TH Polyclonal Antibody (144-00028)

Expiration:

12 months from the date of shipment when stored properly.

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