Anti-THBS2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P35442
Gene Id	7058
Gene Symbols	THBS2
Protein Name / Synonyms	THBS2, TSP2, thrombospondin-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

129kDa

Observed MW

150kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human THBS2 (NP_003238.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using THBS2 antibody (144-08561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - THBS2 antibody (144-08561)

Immunohistochemistry of paraffin-embedded human stomach using THBS2 antibody (144-08561) at dilution of 1:100 (40x lens).
Immunohistochemistry - THBS2 antibody (144-08561)

Expiration:

12 months from the date of shipment when stored properly.

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