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Anti-TIRAP Antibody
Catalog #: 144-12606
TIRAP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P58753 |
| Gene Id | 114609 |
| Gene Symbols | TIRAP |
| Protein Name / Synonyms | TIRAP, BACTS1, Mal, MyD88-2, wyatt, TIR domain containing adaptor protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation, Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
23kDa/25kDa/27kDa
Observed MW
32kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human TIRAP (NP_001034750.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TIRAP antibody (144-12606) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse kidney using TIRAP antibody (144-12606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using TIRAP antibody (144-12606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using TIRAP antibody (144-12606) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TIRAP antibody (144-12606) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.