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Anti-TKT Antibody
Catalog #: 144-06314
TKT Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P29401 |
| Gene Id | 7086 |
| Gene Symbols | TKT |
| Protein Name / Synonyms | TKT, HEL-S-48, HEL107, SDDHD, TK, TKT1, transketolase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
67kDa/68kDa
Observed MW
68kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TKT (NP_001128527.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TKT antibody (144-06314) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using TKT antibody (144-06314). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.