Anti-TLK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q86UE8
Gene Id	11011
Gene Symbols	TLK2
Protein Name / Synonyms	TLK2, HsHPK, PKU-ALPHA, tousled like kinase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

82kDa/85kDa/87kDa

Observed MW

87kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TLK2 antibody (144-06770) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - TLK2 antibody (144-06770)

Immunohistochemistry of paraffin-embedded human liver injury using TLK2 antibody (144-06770) at dilution of 1:100 (40x lens).
Immunohistochemistry - TLK2 antibody (144-06770)

Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody (144-06770) at dilution of 1:100 (40x lens).
Immunohistochemistry - TLK2 antibody (144-06770)

Immunofluorescence analysis of U2OS cells using TLK2 antibody (144-06770). Blue: DAPI for nuclear staining.
Immunofluorescence - TLK2 antibody (144-06770)

Expiration:

12 months from the date of shipment when stored properly.

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