Anti-TLR6 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9Y2C9
Gene Id	10333
Gene Symbols	TLR6
Protein Name / Synonyms	TLR6 (C-term)
Clonality	Polyclonal
Preparation	This TLR6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-423 amino acids from the C-terminal region of human TLR6.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

TLR6, a Type I membrane protein that belongs to the Toll-like rceptor family, participates in the innate immune response to Gram-positive bacteria and fungi. It acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. The protein recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. It binds to TLR2 via their respective extracellular domains, and to MyD88 via their respective TIR domains. TLR6 is detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.

Specificity

Other Names

TLR6; Toll-like receptor 6; CD_antigen=CD286; Flags: Precursor

NCBI Accession #

NP_006059.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

91880

Recommended Dilutions

FC: 1:10-50; WB: 1:1000; IHC: 1:50-100

	Flow cytometric analysis of NCI-H460 cells using TLR6 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of anti-TLR6 Pab (Cat# 102-12776) in mouse liver tissue lysate (35ug/lane). TLR6(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with TLR6 antibody (C-term) (Cat# 102-12776), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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